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. 2017 Jun 8;13(8):1318–1330. doi: 10.1080/15548627.2017.1327103

Table 3.

Oligonucleotide primers used for plasmid construction in this study.

Gene (Locus) Description Enzyme sites Primer sequence
GCN5(MGG__03677) Deletion construct 5′-AATGTGAATTCGAACACAAAACGTTCAG-3′
    5′-GGAACGGGATCCGTGCTATCTCGTTGGG-3′
    PstI 5′-GACTGTTCTGCAGTTTGCTGACCCATTTGTACATGAT-3′
    HindIII 5′-GAGTGTTAAGCTTCTAGGCGTCGGATCACAGTGCGCAT-3′
  GFP-tagging (N-terminal) under RP27 promoter BamHI 5′-GAGAGTGGATCCATAAATGTAGGTATTACCTGTA-3′
    NcoI 5′-GAGAGTGACCATGGTTTGAAGATTGGGTTCCTACGA-3′
    NcoI 5′-GAGAGTGACCATGGTGAGCAAGGGCGAGGAGCTGT-3′
    KpnI 5′-GAGAGTGAGGTACCCTTGTACAGCTCGTCCATGCCGAG-3′
    KpnI 5′-GAGAGTGAGGTACCTCTACAGCAACAGAAGATAGTACAAATGGC-3′
    EcoRI 5′-GTGTGAATTCTGCGCATTAAAGTCATGGTTCAAGATGTGA-3′
  mCherry-tagging (C-terminal) EcoRI 5′-GTGTGAATTCGAGGGCGGCACCATAATGCAGTGCA-3′
    NdeI 5′-GGAATTCCATATGTGGTTCAAGATGTGACCACTCGGGG-3′
    NdeI 5′-GGAATTCCATATGAAGGGCGAGGAGGATAACATGG-3′
    BamHI 5′-GTGTGGATCCCTACTTGTACAGCTCGTCCATGCCG-3′
  Complementation EcoRI 5′-GAGTGAGAATTCGTTCAGTTTCGGGGAATCTTACGCC-3′
    KpnI 5′-AGAGTGAGGTACCCAAACCAACTATATGCATGTTCTCAAAAGCCGAC-3′
ATG1 (MGG_06393) Deletion construct EcoRI 5′-GTGTGAATTCAAGCCTCCAGATACAGAGGTTCTGT-3′
    BamHI 5′-GTGTGGATCCCTCAACCGGGTATTGTTTCATCTT-3′
    PstI 5′-GTGTCTGCAGAAGCGAGACGGTTGGCCCCCTAA-3′
    HindIII 5′-GTGTAAGCTTAAGCCTCCAGATACAGAGGTTCTGT-3′
ATG7 (MGG_07297) dsRed-tagging (C-terminal), under RP27 promoter NcoI 5′-AACCCAATCTTCAAACCATGGGAAATGATGAGGCGGCCG-3′
    EcoRI 5′-CTATGACATGATTACGAATTCAAGCATCTCACCATCCCCTTC-3′
    EcoRI 5′-CCGGAATTCATGGACAACACCGAGGACGTCATCAAG-3′
    EcoRI 5′-CCGGAATTCGAAACGCGTTTTATTCTTGTTGACATGGAGCTATTAAATCACTACTGGGAGCCGGAGTGGCGGG-3′