Table 3.

Oligonucleotide primers used for plasmid construction in this study.

Gene (Locus)	Description	Enzyme sites	Primer sequence
GCN5(MGG__03677)	Deletion construct	—	5′-AATGTGAATTCGAACACAAAACGTTCAG-3′
		—	5′-GGAACGGGATCCGTGCTATCTCGTTGGG-3′
		PstI	5′-GACTGTTCTGCAGTTTGCTGACCCATTTGTACATGAT-3′
		HindIII	5′-GAGTGTTAAGCTTCTAGGCGTCGGATCACAGTGCGCAT-3′
	GFP-tagging (N-terminal) under RP27 promoter	BamHI	5′-GAGAGTGGATCCATAAATGTAGGTATTACCTGTA-3′
		NcoI	5′-GAGAGTGACCATGGTTTGAAGATTGGGTTCCTACGA-3′
		NcoI	5′-GAGAGTGACCATGGTGAGCAAGGGCGAGGAGCTGT-3′
		KpnI	5′-GAGAGTGAGGTACCCTTGTACAGCTCGTCCATGCCGAG-3′
		KpnI	5′-GAGAGTGAGGTACCTCTACAGCAACAGAAGATAGTACAAATGGC-3′
		EcoRI	5′-GTGTGAATTCTGCGCATTAAAGTCATGGTTCAAGATGTGA-3′
	mCherry-tagging (C-terminal)	EcoRI	5′-GTGTGAATTCGAGGGCGGCACCATAATGCAGTGCA-3′
		NdeI	5′-GGAATTCCATATGTGGTTCAAGATGTGACCACTCGGGG-3′
		NdeI	5′-GGAATTCCATATGAAGGGCGAGGAGGATAACATGG-3′
		BamHI	5′-GTGTGGATCCCTACTTGTACAGCTCGTCCATGCCG-3′
	Complementation	EcoRI	5′-GAGTGAGAATTCGTTCAGTTTCGGGGAATCTTACGCC-3′
		KpnI	5′-AGAGTGAGGTACCCAAACCAACTATATGCATGTTCTCAAAAGCCGAC-3′
ATG1 (MGG_06393)	Deletion construct	EcoRI	5′-GTGTGAATTCAAGCCTCCAGATACAGAGGTTCTGT-3′
		BamHI	5′-GTGTGGATCCCTCAACCGGGTATTGTTTCATCTT-3′
		PstI	5′-GTGTCTGCAGAAGCGAGACGGTTGGCCCCCTAA-3′
		HindIII	5′-GTGTAAGCTTAAGCCTCCAGATACAGAGGTTCTGT-3′
ATG7 (MGG_07297)	dsRed-tagging (C-terminal), under RP27 promoter	NcoI	5′-AACCCAATCTTCAAACCATGGGAAATGATGAGGCGGCCG-3′
		EcoRI	5′-CTATGACATGATTACGAATTCAAGCATCTCACCATCCCCTTC-3′
		EcoRI	5′-CCGGAATTCATGGACAACACCGAGGACGTCATCAAG-3′
		EcoRI	5′-CCGGAATTCGAAACGCGTTTTATTCTTGTTGACATGGAGCTATTAAATCACTACTGGGAGCCGGAGTGGCGGG-3′