Table 3.
Oligonucleotide primers used for plasmid construction in this study.
Gene (Locus) | Description | Enzyme sites | Primer sequence |
---|---|---|---|
GCN5(MGG__03677) | Deletion construct | — | 5′-AATGTGAATTCGAACACAAAACGTTCAG-3′ |
— | 5′-GGAACGGGATCCGTGCTATCTCGTTGGG-3′ | ||
PstI | 5′-GACTGTTCTGCAGTTTGCTGACCCATTTGTACATGAT-3′ | ||
HindIII | 5′-GAGTGTTAAGCTTCTAGGCGTCGGATCACAGTGCGCAT-3′ | ||
GFP-tagging (N-terminal) under RP27 promoter | BamHI | 5′-GAGAGTGGATCCATAAATGTAGGTATTACCTGTA-3′ | |
NcoI | 5′-GAGAGTGACCATGGTTTGAAGATTGGGTTCCTACGA-3′ | ||
NcoI | 5′-GAGAGTGACCATGGTGAGCAAGGGCGAGGAGCTGT-3′ | ||
KpnI | 5′-GAGAGTGAGGTACCCTTGTACAGCTCGTCCATGCCGAG-3′ | ||
KpnI | 5′-GAGAGTGAGGTACCTCTACAGCAACAGAAGATAGTACAAATGGC-3′ | ||
EcoRI | 5′-GTGTGAATTCTGCGCATTAAAGTCATGGTTCAAGATGTGA-3′ | ||
mCherry-tagging (C-terminal) | EcoRI | 5′-GTGTGAATTCGAGGGCGGCACCATAATGCAGTGCA-3′ | |
NdeI | 5′-GGAATTCCATATGTGGTTCAAGATGTGACCACTCGGGG-3′ | ||
NdeI | 5′-GGAATTCCATATGAAGGGCGAGGAGGATAACATGG-3′ | ||
BamHI | 5′-GTGTGGATCCCTACTTGTACAGCTCGTCCATGCCG-3′ | ||
Complementation | EcoRI | 5′-GAGTGAGAATTCGTTCAGTTTCGGGGAATCTTACGCC-3′ | |
KpnI | 5′-AGAGTGAGGTACCCAAACCAACTATATGCATGTTCTCAAAAGCCGAC-3′ | ||
ATG1 (MGG_06393) | Deletion construct | EcoRI | 5′-GTGTGAATTCAAGCCTCCAGATACAGAGGTTCTGT-3′ |
BamHI | 5′-GTGTGGATCCCTCAACCGGGTATTGTTTCATCTT-3′ | ||
PstI | 5′-GTGTCTGCAGAAGCGAGACGGTTGGCCCCCTAA-3′ | ||
HindIII | 5′-GTGTAAGCTTAAGCCTCCAGATACAGAGGTTCTGT-3′ | ||
ATG7 (MGG_07297) | dsRed-tagging (C-terminal), under RP27 promoter | NcoI | 5′-AACCCAATCTTCAAACCATGGGAAATGATGAGGCGGCCG-3′ |
EcoRI | 5′-CTATGACATGATTACGAATTCAAGCATCTCACCATCCCCTTC-3′ | ||
EcoRI | 5′-CCGGAATTCATGGACAACACCGAGGACGTCATCAAG-3′ | ||
EcoRI | 5′-CCGGAATTCGAAACGCGTTTTATTCTTGTTGACATGGAGCTATTAAATCACTACTGGGAGCCGGAGTGGCGGG-3′ |