Figure 4.

Accumulated LC3-positive structures did not colocalize with phagophore and lysosome markers in DOX-treated TetON-GFPSTX17ΔNTD and TetON-GFPSTX17FL HeLa cells. (A-C) TetON-GFPSTX17ΔNTD and TetON-GFPSTX17FL HeLa cells were cultured with DOX (1.5 μg/ml) for 2 d. Cells were cultured in regular (Gr) or starvation (St) medium for 1 h and analyzed by immunofluorescence microscopy using antibodies against RB1CC1 (A), WIPI2 (B), and LAMP1 (C). Colocalization analysis was performed as described in Materials and Methods. Each correlation plot is derived from 30–61 cells in 3 different fields of view. The mean Pearson's correlation coefficient values ± SEM are shown on the plots and the graphs. The intensities of GFP-STX17 variants are represented on the x-axis. Asterisks indicate significant differences between MYC-tagged STX17 variants at p < 0.01 by t-test. Scale bars: 10 μm; 2 μm in insets.