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. 2001 Aug 15;29(16):3439–3447. doi: 10.1093/nar/29.16.3439

Figure 2.

Figure 2

HDAC4 inhibits C2C12 differentiation and MEF2 expression. (A) C2C12 myoblasts were transfected either with pcHDAC4-Flag, pBJ5-HDAC5-Flag, pcHDAC1-Flag or pcGAL4 and pEGFP as a transfection marker (100 ng each plasmid). After transfection cells were transferred to differentiation medium for 4 days before fixing. GFP staining was visualised using confocal microscopy. (B) C2C12 myoblasts were transfected with 1 µg 3×MCK-MEF2-luc and 200 ng either pcHDAC4-Flag or pcHDAC1-Flag. After transfection cells were transferred to differentiation medium for 2 days. Cell extracts were prepared and analysed for luciferase activity.