Table S1.
Inclusion and exclusion criteria for study subjects
| pCAP group | sCAP group | Pulpitis group | Abscess group | Control group |
|---|---|---|---|---|
| Inclusion criteria | ||||
| Age at least 10 years | Age at least 10 years | Age at least 10 years | Age at least 10 years | Age at least 10 years |
| Tooth with AP lesion without RCF | Tooth with AP lesion with RCF | Tooth without AP lesion | Tooth with AP lesion | Excellent oral health |
| Objective and subjective criteria for AP | Objective and subjective criteria for AP | Objective and subjective criteria for pulpitis | Objective and subjective criteria for abscess of endodontic origin | Excellent oral hygiene |
| Lesion on X-ray with PAI >2 | Lesion on X-ray with PAI >2 | No lesion on X-ray (PAI<2) | Lesion on X-ray with PAI >2 | Intact teeth |
| Excellent general health | Excellent general health | Excellent general health | Good general health | Excellent general health |
| Exclusion criteria | ||||
| Age <10 years | Age <10 years | Age <10 years | Age <10 years | Age <10 years |
| Diseased primary teeth | Diseased primary teeth | Diseased primary teeth | Diseased primary teeth | Bad oral hygiene |
| Vertical root fracture | Vertical root fracture | Vertical root fracture | Vertical root fracture | Any kind of tooth disease |
| Horizontal root fracture | Horizontal root fracture | Horizontal root fracture | Horizontal root fracture | Any kind of gingival or periodontal disease |
| Tooth with endo-perio lesion | Tooth with endo-perio lesion | Tooth with endo-perio lesion | Tooth with endo-perio lesion | Restored teeth |
| Tooth with peri-endo lesion | Tooth with peri-endo lesion | Tooth with peri-endo lesion | Tooth with peri-endo lesion | Any kind of general acute disease |
| Other conditions and clinical situations that require RCT | Other conditions and clinical situations that require RCT | Other conditions and clinical situations that require RCT | Other conditions and clinical situations that require RCT | Any kind of chronic general disease |
| Tooth with gingival pocket >3.5 mm | Tooth with gingival pocket >3.5 mm | Tooth with gingival pocket >3.5 mm | Tooth with gingival pocket >3.5 mm | Any kind of infectious disease in present or past history |
| Unrestorable tooth (deeply fractured or highly carious tooth which is impossible to restore and therefore must be extracted) | Unrestorable tooth (deeply fractured or highly carious tooth which is impossible to restore and therefore must be extracted) | Unrestorable tooth (deeply fractured or highly carious tooth which is impossible to restore and therefore must be extracted) | Unrestorable tooth (deeply fractured or highly carious tooth which is impossible to restore and therefore must be extracted) | |
| Tooth that has undergone apical surgery with or without retrograde filling | Tooth that has undergone apical surgery with or without retrograde filling | Tooth that has undergone apical surgery with or without retrograde filling | ||
| Any type of smoking or tobacco abuse | Any type of smoking or tobacco abuse | Any type of smoking or tobacco abuse | Any type of smoking or tobacco abuse | Any type of smoking or tobacco abuse |
| Antibiotic or any other medicament usage in previous 3 months | Antibiotic or any other medicament usage in previous 3 months | Antibiotic or any other medicament usage in previous 3 months | Antibiotic or any other medicament usage in previous 3 months | Antibiotic or any other medicament usage in previous 6 months |
| Diabetes I or II | Diabetes I or II | Diabetes I or II | Diabetes I or II | Diabetes I or II |
| Immune suppressed person (HIV, AIDS, chemotherapy) | Immune suppressed person (HIV, AIDS, chemotherapy) | Immune suppressed person (HIV, AIDS, chemotherapy) | Immune suppressed person (HIV, AIDS, chemotherapy) |
Abbreviations: HIV, human immunodeficiency virus; AIDS, acquired immunodeficiency syndrome; pCAP, primary chronic apical periodontitis; sCAP, secondary chronic apical periodontitis; AP, apical periodontitis; RCF, root canal filling; RCT, root canal treatment; PAI, periapical index.