Fig 3. Hepatic inflammation in WT and CD18-mutant mice in response to MCD feeding.

(A) Gr-1 staining and quantitation of Gr1-positive cells in WT and CD18 mut liver at 3 wk. Photomicrographs illustrate that Gr-1-positive cells are abundant in both WT and CD18-mutant (CD18 mut) mice after MCD feeding, although in different distributions. Original magnification 15X. Histograms illustrated Gr1-positive cell counts in WT and CD18 mut livers, performed as described in Methods. Values represent mean ± SE for n = 5. (B) Representative FACS plots of hepatic leukocytes from mice fed chow or MCD diets. MCD feeding enhances the proportion of neutrophils (Ly6G^high) and inflammatory monocytes (Ly6C^high), while the lack of CD18 further enhances the accumulation of neutrophils but not inflammatory monocytes. Data are illustrative of n = 6 mice per group (24 per cohort), performed as 2 replicate experiments involving 12 mice each (3 per group).