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. Author manuscript; available in PMC: 2017 Sep 5.
Published in final edited form as: Nat Immunol. 2011 Jun 12;12(7):616–623. doi: 10.1038/ni.2051

Figure 2.

Figure 2

Jα18−/− CD1d–α-GalCer+ NKT cells express a semi-invariant Vα10-Jα50–Vβ8+ TCR. (a) PCR analysis of cDNA isolated from CD1d–α-GalCer-reactive cells sorted from BALB/c Jα18−/− thymuses and amplified with a panel of primers specific for each TCRα V-gene segment or the α-chain constant region (Cα). – (far right), Cα primers with no cDNA. (b) Single-cell PCR analysis for Vα10 on cDNA isolated from Vβ8.1 and Vβ8.2+ cells positive for CD1d–α-GalCer tetramer (right; Jα18−/− mouse–derived) or Vβ8.1 and Vβ8.2+ CD4+ αβTCR+ cells negative for the CD1d–α-GalCer tetramer (left; conventional T cells) sorted from BALB/c Jα18−/− thymuses; n = 16 cells per panel. (c) Staining of surface TCRβ (top row) and unloaded or α-GalCer-loaded CD1d tetramer (bottom row) on green fluorescent protein–gated human epithelial 293T cells transfected to express full-length rearranged Vα10-Jα50 or Vα14-Jα18 TCR α-chain, plus Vβ8.1, Vβ8.3 or Vβ7 TCR β-chain, and CD3 complex. Isotype, isotype-matched control antibody. Numbers above bracketed lines indicate percent-positive cells. Data are from one experiment (a,b; one for each) or are representative of one (Vβ8.1) or two (Vβ8.3 and Vβ7) experiments (c).