Fig 4. Effect of PFC/carbogen and carbogen alone on hypoxia in intracranial mouse glioma tumours.

Animals were divided into groups of 5 mice each. A. Untreated control mice; B. Mice exposed to PFC emulsions; C. Mice exposed to carbogen and D. Mice exposed to both PFC emulsions and carbogen. Tumor-bearing mice were injected IV with 1.5cc/kg of a 40% PFC emulsion and hypoxia was measured using pimonidazole-HCL (PIM). Mice that received carbogen (95%O₂/5%CO₂) for 1h were placed in an air-tight chamber. Photos are representative of 3 separate experiments.