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. 2017 Sep 5;12(9):e0183897. doi: 10.1371/journal.pone.0183897

Fig 3. ZDF subcutaneous adipocytes co-culture negatively affects MCF7 cell cycle proteins in does-dependent manner.

Fig 3

A: Representative western blots for selected proteins showing the effects of treatment with UT (open bar) or ZDF scAT increasing adipocyte number (closed bars) or the highest adipocyte number (1.6×105) plus ADIPO (18 nM; grey bar) co-culture treatment for 24 hours in arrested MCF7 cells. B-F: Graphical representations of multiple experiments showing the effects of ZDF scAT increasing adipocyte number and the addition of ADIPO (18 nM) on pAMPKT172, pAKTT308, p27, p27T198 and AdipoR1 protein levels. β-actin was used as a loading control. One-way ANOVAs showed significant main effects for all experiments (p<0.05). * denotes significantly different from ZDF (p<0.05) as determined by Tukey’s post-hoc tests (n = 5/group).