Fig 8. Quantification of epimastigotes intracellular levels of calcium (A) and superoxide anion (B) upon JG and Col1.7G2 epimastigotes treatment with H₂O₂.

(A) JG and Col1.7G2 cells previously incubated with fura 2-AM, were treated or not (control) with 30 μM H₂O₂ for 30 minutes and the calcium levels in 10⁷ parasites/mL was determined. (B) JG and Col1.7G2 parasites were incubated with MitoSOX and subsequently exposed to 30 μM H₂O₂ for 30 minutes, the oxMitoSOX level was measured in 10⁷ parasites/mL. The data represent the mean of triplicates ± the standard error of the mean (SEM). Asterisks indicate statistically significant differences (**<0.01 *** p≤0.001—Student's t-test).