Fig 4. Tn-seq analyses of M1T1 GAS 5448 fitness during murine skin and soft tissue infection.

Genome-scale summary of the ratios of mutant abundance (log₂FC, Y axis) calculated using DEseq2 for each gene (X axis) comparing the GAS 5448 Krmit library composition retrieved from in vivo lesions after 12, 24 and 48 HPI versus the T₀ input pools (A, B and C, respectively). Gene mutations conferring decreased (log₂FC < -1, p < 0.05) or increased fitness (log₂FC > 1, p < 0.05) are indicated with orange and green circles, respectively. Neutral mutations (grey circles) and essential genes (black circles) are also indicated.