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. 2017 Sep 5;6:e23770. doi: 10.7554/eLife.23770

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© 2017, Tran et al

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Figure 3. — Carboxylates were extracted from species of Streptomyces and labeled with a pyridinium moiety for easier detection by mass spectrometry. For example, to detect dodecanoate after Streptomyces cell-free supernatants were extracted and concentrated, dodecanoate and the other carboxylates in the extract were converted to pyridinium esters by treatment with carbonyldiimidazole (CDI) in acetonitrile (ACN).

Figure 3—figure supplement 1. — Carboxylates were extracted from species of Streptomyces and labeled with a pyridinium moiety for easier detection by mass spectrometry. For example, to detect dodecanoate after Streptomyces cell-free supernatants were extracted and concentrated, dodecanoate and the other carboxylates in the extract were converted to pyridinium esters by treatment with carbonyldiimidazole (CDI) in acetonitrile (ACN).