Video 1. ASAP2s optical response to cardiac APs in a human embryonic stem cell-derived cardiomyocyte (hESC-CM).
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DOI: 10.7554/eLife.25690.011
A hESC-CM was transfected with ASAP2s at 27 days post-differentiation and was imaged three days later at 100 Hz and with a power density of 11 mW/mm2 at the sample plane. Quantification of the fluorescence response during the first 10 s is shown in Figure 2B. The movie corresponds to a single trial without filtering, smoothing, or photobleaching correction.