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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jun 9;1060:158–165. doi: 10.1016/j.jchromb.2017.06.017

Table 2.

Estimated recovery of 25-OHD3-3-sulfate and 25-OHD3-3-glucuronide and their deuterated internal standards following solid phase extraction

25-OHD3-3-sulfate d6-25-OHD3-3-sulfate 25-OHD3-3-glucuronide d6-25-OHD3-3-glucuronide
10 nM 180 nM ~ 20 nM 1 nM 18 nM ~ 8 nM
99.6 91.2 95.9 114 98.4 107
99.8 88.8 91.8 115 98.0 72.3
Recovery (%)a 94.8 91.6 98.0 111 100 111
96.2 89.3 96.2 113 102 104
98.3 92.4 106 111 104 90.4
93.1 93.6 101 117 103 116
N 6 6 6 6 6 6
Average 97.0 91.2 98.2 113 101 100
SD (%) 2.7 1.9 4.9 2.4 2.5 16.2
a

Recovery (%) was calculated as the percentage of the peak area of samples post-extraction to the peak area without extraction.