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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jun 9;1060:158–165. doi: 10.1016/j.jchromb.2017.06.017

Table 3.

Matrix effects of vitamin D3-free human serum on 25-OHD3-3-sulfate and 25-OHD3-3-glucuronide and their deuterated internal standards

25-OHD3-3-sulfate
25-OHD3-3-glucuronide
Analyte (10 nM) IS Analyte (180 nM) IS Analyte (1 nM) IS Analyte (18 nM) IS
228 207 171 204 182 202 154 207
Matrix effect (%)a 217 218 176 148 196 165 158 124
223 189 178 164 194 210 164 140
N 3 3 3 3 3 3 3 3
Average 223 205 175 172 191 192 159 159
SD (%) 5.1 14.6 4.0 28.8 7.6 24.1 5.0 5.0
a

The serum matrix effect (%) was calculated as the percentage of the peak area in the presence of matrix to the peak area in the absence of matrix.