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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jun 9;1060:158–165. doi: 10.1016/j.jchromb.2017.06.017

Table 4.

Stability of 25-OHD3-3-sulfate and 25-OHD3-3-glucuronide in human serum under various treatment conditions

Accuracy (%)a 25-OHD3-3-sulfate 25-OHD3-3-glucuronide
10 nM 180 nM 1 nM 18 nM
Prepared sample in autosampler at 4°C, 24 hr 96.5 ± 3.3 92.6 ± 3.5 114 ± 3 95.9 ± 3.1
Plasma at room temperature, 2 hr 93.8 ± 2.6 89.3 ± 3.4 109 ± 38 89.1 ± 3.8
Plasma following three freeze-thaw cycles 89.8 ± 0.9 96.1 ± 4.0 92.8 ± 12.4 90.9 ± 2.2
Plasma stored at −80°C, 50 days 90.8 ± 1.6 91.7 ± 0.6 92.4 ± 0.2 90.6 ± 1.0
a

Accuracy (%) was calculated as the percentage of the determined concentration after the respective treatment to the theoretical concentration. Shown are mean ± SD.