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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jun 19;1060:316–324. doi: 10.1016/j.jchromb.2017.06.028

Table 2.

LC/MS determination of taxane and anthracycline drugs in urine

Parent drug Sample preparation Chromatography Interface/Detection Target analyte m/z of mass transition Limit of Detection Reference
paclitaxel (TAX) LLE
methyl-tert-butyl ether/ACN		 RP C18/gradient
HCOONH4/ACN

4.6 × 50 mm, 1.8μm		 ESI+/QTrap TAX
d5-PA		 854/105
859/105		 0.05 μg/L [23]
paclitaxel
  docetaxel (DE)		 LLE
methyl-tert-butyl ether/aqueous MeOH		 RP C18/isocratic
MeOH/NH4OH

2.1 × 150 mm, 5μm		 ESI/QQQ/MRM+ TAX
13C6-PA
DE
d9-DE		 854/509
860/515
808/527
817/527		 0.5 μg/L
0.5 μg/L		 [32]
   anthracyclines
epirubicin
   doxorubicin
    daunoribicin
idarubicin		 SPE C18
CH2Cl2/2-propanol		 RP C8/gradient
HCOOH/ACN/MeOH

4.6 × 150 mm, 5μm		 ESI/QQQ/MRM+ EPI
DXR
DNR
IDA
epi-IDA		 544/321
544/397
528/321
498/291
528/321		 0.04 μg/L
0.04 μg/L
0.01 μg/L
0.01 μg/L		 [33]

Legend of abbreviations used in Table 2. LC/MS determination of taxane and anthracycline drugs in urine.

ACN: acetonitrile, CH2Cl2: methylene chloride, ESI: electrospray ionization, MeOH: methanol, LLE: liquid-liquid extraction, MRM: multiple reaction monitoring, QQQ: triple quadrupole, RP: reversed phase, SPE: solid phase extraction, SRM: single reaction monitoring