Table 4.
LC/MS simultaneous determination of antineoplastic drugs in urine
| Parent drug | Sample preparation | Chromatography | Interface/Detection | Target analyte | m/z or mass transition | Limit of detection | Reference |
|---|---|---|---|---|---|---|---|
| multiple determination | ABN-SPE 96-well CHOOH/MeOH |
RP C12/gradient CH3CO2NH4/MeOH |
ESI/QQQ/MRM+ | [39] | |||
| 0.5 × 50 mm, 4μm | |||||||
| cyclophosphamide | CP TRP |
261/140 323/154 |
0.04 μg/L | ||||
| methotrexate | MTX | 455/308 | 0.2 μg/L | ||||
| multiple determination | SPE C18 CH2Cl2/2-propanol/MeOH |
RP C8, gradient CHOOH/ACN |
ESI/QQQ/MRM+ | [40] | |||
| 4.6 × 150 mm, 5μm | |||||||
| cyclophosphamide | CP TRP |
261/140 323/154 |
0.1 μg/L | ||||
| ifosphosphamide | IF | 261/92 | 0.1 μg/L | ||||
| doxorubicin | DXR | 528/321 | 0.2 μg/L | ||||
| epirubicin | EPI | 544/397 | 0.1 μg/L | ||||
| daunorubicin | DNR EDR |
528/321 528/321 |
0.1 μg/L | ||||
| multiple determination | dispersive SPE RP sorbent aqueous MeOH |
RP PFP/gradient CHOOH/ACN |
ESI/QTrap/SRM+ | [41] | |||
| 2.1 × 100 mm, 2.6 μm | |||||||
| cyclophosphamide | CP 2H4 -CP |
261/106 261/140 |
0.33 μg/L | ||||
| ifosphosphamide | IF 2H4 -IF |
261/154 261/92 |
0.13 μg/L | ||||
| doxorubicin | DXR 13C,2H3-DXR |
544/379 544/397 |
0.07 μg/L | ||||
| epirubicin | EPI 13C,2H3-EPI |
544/379 544/397 |
0.03 μg/L | ||||
| 5-fluorouracil | 5-FU 13C2,15N2-5-FU |
129/86 129/59 |
33.33 μg/L | ||||
| cytarabine | CYT 13C3-CYT |
244/95 244/112 |
0.33 μg/L | ||||
| gemcitabine | GCA 13C2,15N2- GEM |
264/95 264/112 |
0.67 μg/L | ||||
| dacarbazine | DAC 2H6 -DAC |
183/123 183/166 |
0.67 μg/L | ||||
| etoposide | ETOP 13C,2H3-ETOP |
589/425 589/229 |
0.17 μg/L | ||||
| methotrexate | MTX 2H3 -MTX |
455/134 455/308 |
0.01 μg/L | ||||
| vinblastine | VBL 2H3 -VBL |
811/751 8///224 |
0.83 μg/L | ||||
| vincristine | VCR 2H3 -VCR |
413/362 413/392 |
0.17 μg/L | ||||
| paclitaxel | TAX 2H5 -TAX |
854/569 854/286 |
0.33 μg/L |
Legend of abbreviations used in Table 4. LC/MS simultaneous determination of antineoplastic drugs in urine
ABN-SPE: Acid Base Neutral SPE, ACN: acetonitrile, CH2Cl2: methylene chloride, CHOOH: formic acid, EDR: epidaunorubicin, ESI: electrospray ionization, MeOH: methanol, MRM: multiple reaction monitoring, QQQ: triple quadrupole, RP: reversed phase, PFP: pentafluorophenyl, SPE: solid phase extraction, SRM: single reaction monitoring, TRP: trophosphamide