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Differential plasmid DNA digestion from E. coli. An E. coli dam mutant (Dam::16KM) or its parental strain (MG1655) were complemented with either a plasmid harboring the P. luminescens dam gene (Dam) or the pBBR1-MCS5 empty vector (MCS5). Plasmid DNA was extracted and digested by MboI (active on unmethylated 5′-GATC-3′ sites) or DpnI (active on 5′-GmeATC-3′ sites). pBBR1-MCS5 (4,768 bp) has 17 GATC sites and pBB-Dam (5,618 bp) has 19 GATC sites.
