Table 1.
Strains and plasmids used in this work.
| Strain or plasmid | Relevant genotype and characteristicsa | Reference or source |
|---|---|---|
| STRAINS | ||
| Photorhabdus luminescens TT01 | Wild type | Duchaud et al., 2003 |
| Escherichia coli XL1 blue MRF' | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Agilent Technologies |
| E. coli WM3064 | thrB1004 pro thi rpsl hsdS lacZΔM15 RP4-1360Δ(araBAD)567 ΔdapA1341::[erm pir (wt)] | Paulick et al., 2009 |
| E. coli MG1655 | Wild type | Lobner-Olesen and von Freiesleben, 1996 |
| E. coli Dam::16KM | MG1655 dam16::Kmr | Lobner-Olesen and von Freiesleben, 1996 |
| Xenorhabdus nematophila F1 | Wild type | Lanois et al., 2013 |
| Micrococcus luteus | Wild type | Pasteur Institute Culture collection, Paris, France |
| PLASMIDS | ||
| pBBR1MCS-5 | Cloning vector, Gmr | Kovach et al., 1995 |
| Ptet-MCS | Cloning vector, Kmr | Jubelin et al., 2013 |
| Ptet-MCS-Dam | 853 pb PCR fragment (dam gene) inserted between KpnI and BamHI site of Ptet-MCS plasmid | This study |
| pBB-Dam | 864 pb fragment (RBS and dam gene) isolated from Ptet-MCS-Dam and inserted between EcoRI and BamHI site of pBBR1MCS-5 plasmid | This study |
| pJQ200KS | Mobilizable vector, Gmr | Quandt and Hynes, 1993 |
| pJQ-Δdam | Region overlapping the dam gene disrupted by a Cmr cassette and inserted between PstI and XbaI site of pJQ200KS plasmid | This study |
a
Km, kanamycin; Gm, gentamicin; Cm, chloramphenicol.