Figure 2.

IL-12-secreting CAR T cells prolong survival in an advanced tumor model of ovarian peritoneal carcinomatosis and is dependent on autocrine IL-12 signaling. (a) Female wild type C57BL/6 mice (WT) between 6–8 weeks old were inoculated with 1 × 10⁷ ID8-Muc16^ecto cells i.p and treated 35 days later with 2 × 10⁶ indicated CAR T cells i.p., *p = 0.02. Number of mice per group: 5 (1928ζ), 5 (1928ζ-IL12), 6 (4H1128ζ), 8 (4H1128ζ-IL12). (b) Female WT mice between 6–8 weeks old were inoculated with 1 × 10⁷ ID8-Muc16^ecto cells i.p and treated 35 days later with 2 × 10⁶ CAR T cells (i.p) derived from IL-12R^−/− splenocytes. Number of mice per group: 5 (1928ζ), 5 (1928ζ-IL12), 5 (4H1128ζ), 5 (4H1128ζ-IL12). (c) Female WT mice between 6–8 weeks old were inoculated with 1 × 10⁷ ID8-Muc16^ecto cells i.p and treated 42 days later with 2 × 10⁶ indicated CAR T cells i.p, *p < 0.01. Number of mice per group: 5 (1928ζ), 5 (1928ζ-IL12), 6 (4H1128ζ), 8 (4H1128ζ-IL12). Results are pooled from 2 independent experiments. Statistical analysis performed using a log-rank (Mantel-Cox) test.