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. 2017 Sep 5;7:10527. doi: 10.1038/s41598-017-10317-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative transverse sections of the left proximal tibia in ovariectomized Control (C), Longshanks 1 (LS1) and Longshanks 2 (LS2) mice. The sections were taken from uCT scans at week 0 (Wk 0) and week 6 (Wk 6) of the experiment, starting ~0.18 mm distal to the end of the growth plate, and extending 0.625 mm distally (50 slices). For orientation, the projection at the bottom left of each section is the tibial tuberosity, while the two rounded projections at the top right are the caudal aspects of the tibial condyles. Cortical bone is shown in gray, trabecular bone in green. Note the more sparse, thinner trabeculae in Longshanks mice compared with Control, the relatively greater loss of trabecular bone in ovariectomized Control mice, as well as the relatively thinner cortex in LS1. Not to scale.