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. 2017 Sep 5;7:10409. doi: 10.1038/s41598-017-09847-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Numerical simulations on the 3D reconstruction of a 64 × 64 × 64 voxel vesicle model from 71 noisy projections using GENFIRE, EST, FBP and SIRT. (a–c) Three 10-voxel-thick central slices of the vesicle model in the XY, ZX and ZY planes, respectively. The corresponding three reconstructed slices with GENFIRE (d–f), EST (g–i), FBP (j–l), and SIRT (m–o), where the missing wedge axis is along the z-axis. (p) The FSC between the reconstructions and the model, showing that GENFIRE produces a more faithful reconstruction than other algorithms at all spatial frequencies.