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. 2017 Sep 1;8:1520. doi: 10.3389/fpls.2017.01520

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Copyright © 2017 Zhou, Chen, Khan, Zhao, Chen, Liu, Liao, Kong and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stability ranking and pairwise variation (V) analysis of the 10 candidate reference genes in different sample sets using GeNorm. The average expression stability values (M) of the 10 candidate reference genes performed in all sample (A), 722A sample set (B), 722B sample set (C), The most stable genes are on the right, and the least stable gene are on the left. The optimal number of reference genes required for accurate normalization (D). GeNorm was used to analyze pairwise variation (V_n/V_n+1) between the normalization factors NF_n and NF_n+1, V-value < 0.15 denotes that additional reference genes will not significantly improve the accuracy of the results.