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. 2017 Sep 1;8:1520. doi: 10.3389/fpls.2017.01520

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Copyright © 2017 Zhou, Chen, Khan, Zhao, Chen, Liu, Liao, Kong and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression levels of a target gene, HcPDIL5-2a, at tetrad, monokaryotic and dual-core stage in lines 722A and 722B using different normalization strategies. NF0: the least stable reference gene, TUA; NF1-1: the most stable reference gene, TUB; NF1-2: the most stable two reference gene, TUB and CYP; NF1-3: the most stable three reference gene, TUB, CYP, and PEPKR1; The error bars represent standard deviations of three biological replicates. Significantly up-regulated with respect to control was shown by ^**P < 0.01, and significantly down-regulated was shown ^##P < 0.01.