Table 1.
The amplicon characteristics of primers, 10 candidate reference genes and 6 target genes.
| Gene name (abbreviation) | Accession NO. | Forward primer sequence (5′-3′)/Reverse primer sequence (5′-3′) | PCR Efficiency | R2 | Product size (bp) |
|---|---|---|---|---|---|
| 18S ribosomal RNA (18S) | KX816334 | AGAAACGGCTACCACATC/TACTCATTCCAATTACCAGACTC | 0.953 | 0.999 | 122 |
| Actin 3 (ACT3) | KX689256 | GTGAGGATATTCAACCCCTTGTCT/CATCTTTCTGTCCCATACCAACC | 0.983 | 0.999 | 150 |
| Cyclophilin (CYP) | KX689255 | TCATCTGCACCGCCAAAA/CTTTCTCCACGGCTCTCACC | 0.928 | 0.999 | 96 |
| Eukaryotic elongation factor 1-alpha (ELF1A) | KX703003 | GAACATGATCACGGGGACCT/GAGTGAAGGCAAGCAGAGCA | 0.932 | 0.999 | 128 |
| Glucose-6-phosphate dehydrogenase (G6PD) | KX703004 | ACGAATTCTCGAAAAGTAGCCAAG/TCCGAGTCCATCCACCAAG | 0.972 | 0.999 | 141 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | KX783037 | AACGAAAAGGAATACAAGCCAGAG/AAGACCCTCAACAATGCCAAA | 0.924 | 0.999 | 117 |
| Histone 3 (H3) | KX703005 | GTGGAGTCAAGAAGCCTCACAG/ATGGCTCTGGAAACGCAAA | 0.902 | 0.999 | 164 |
| Phosphoenolpyruvate Carboxylase-Related Kinase 1 (PEPKR1) | KX703006 | TGCCATGAGAATCGCCAAC/GGACACCAACCAAAAGCACA | 1.003 | 0.999 | 149 |
| Alpha-tubulin (TUA) | KX703007 | ATTGGCGGAGGTGATGATG/TGGAAGAGTTGGCGGTATGTT | 0.936 | 0.999 | 140 |
| Beta-tubulin (TUB) | KX703008 | TTTTCCGACCCGACAACTTC/AGTTCCGCTCCTTCCGTGT | 0.930 | 0.999 | 85 |
| Protein disulfide isomerase-like5-2a of kenaf (HcPDIL5-2a) | HQ638208 | CGTTGCTCCTGATGTGTCTATTCT/CTGTCTCATTCAAGCCAAAACCT | 0.913 | 0.999 | 110 |
| ATP synthase subunit 1 (atp1) | KC686836 | TAAAAGCGGTAGATAGCCTGG/AATCGCTACATAGACACAATACAA | 0.909 | 0.999 | 173 |
| ATP synthase subunit 4 (atp4) | MF511050 | TCTTCCCATCGCATCCGTCTT/TTATTCTTACCCCACCCGAACCAT | 0.926 | 1.000 | 159 |
| ATP synthase subunit 6 (atp6) | HM535783 | GCTAATCTCTTATTGTTTTCGCGCA/ATAGCATAGTCCAAGCGAAGCCAC | 0.934 | 1.000 | 113 |
| ATP synthase subunit 8 (atp8) | KM281806 | TTCTGGTTATGCCTTTTCTCTT/TGTTTCTTTCTATTCCGCGTGAG | 0.942 | 1.000 | 319 |
| ATP synthase subunit 9 (atp9)a | JX206830 | ATGAATGATAAAGCGCGTGACGAG/CGGTTAGAGCAAAGCCCAAAATG | 0.995 | 1.000 | 208 |
a
The primers sequences of this gene was according to Zhao et al. (2013).