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. 2017 Sep 1;8:988. doi: 10.3389/fimmu.2017.00988

Figure 4.

Figure 4

Systemic sclerosis (SSc) patient serum increased osteogenic differentiation potential of MSCs. (A) Chondrogenic differentiation of MSCs cultured with human serum AB (SAB) or oxidized SAB (SAB400, SAB1000, and SABH2O2; n = 3 for each condition) or SSc patient serum (n = 13) at day 21. Chondrogenesis was assessed by measuring expression level of specific genes: sex-determining region Y-box9 (SOX9), aggrecan (ACAN), and type II collagen variant B (COL2a1Δ2) by RT-qPCR. (B) Representative photographs of MSCs depicted in (C) and stained with Oil Red O. (C) Adipogenic differentiation of MSCs cultured with human SAB or oxidized SAB (SAB400, SAB1000, and SABH2O2; n = 3 for each condition) or SSc patient serum (n = 13) at day 21. Adipogenesis was assessed by measuring expression level of specific genes: lipoprotein lipase (LPL), peroxisome proliferator-activator receptor (PPAR)-γ, and fatty acid binding protein (FABP)4 by RT-qPCR. (D) Representative photographs of MSCs depicted in (E) and stained with Alizarin Red S. (E) Osteogenic differentiation of MSCs cultured with human SAB or oxidized SAB (SAB400, SAB1000, and SABH2O2; n = 3 for each condition) or SSc patient serum (n = 13) at day 21. Osteogenesis was assessed by measuring expression level of specific genes: Runt-related transcription factor (Runx)2, alkaline phosphatase (AP), and type I collagen (Col I) by RT-qPCR. (F) Representative photographs of MSCs depicted in(E) and stained for AP detection. Data were normalized to 1 for MSCs in SAB-containing medium. *p < 0.05 versus SAB at same time point.