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. 2001 Aug 15;29(16):3311–3319. doi: 10.1093/nar/29.16.3311

Figure 2.

Figure 2

Binding isotherms illustrating the CRE/AP-1 specificities of B70 and variants thereof. Plots show the fraction of CRE24 (open symbols) and AP-123 (closed symbols) bound (F) as a function of peptide concentration at 25°C. Curves represent the best fit of the data to the equation C·{1/(1 + Kapp/[peptide]2)}, where Kapp and C are adjustable parameters. The equation used to determine values for Kapp is independent of whether the bZIP peptide forms a dimer before or after the DNA binding event (58). Each point represents the average of at least three independent determinations. Error bars denote the standard error.