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. 2017 Sep 5;7:10550. doi: 10.1038/s41598-017-10338-6

Figure 4.

Figure 4

MPA reduces the intracellular ATP content. (A) Percentage of ATP in the Jurkat cells incubated with 0.5 µM MPA or 5 µM rapamycin for up to 48 h. The data are from four independent experiments. Mann-Whitney U test: *P < 0.05. (B) Immunoblot representing phospho-AMPKα, AMPKα and actin levels after incubation with 0.5 µM MPA, 5 µM rapamycin or vehicle (V). The immunoblot is representative of three independent experiments. (C) Immunoblot representing LC3 and actin levels after incubation with 0.5 µM MPA, 5 µM rapamycin or vehicle (V). The immunoblot is representative of three independent experiments.