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. 2017 Sep 5;7:10470. doi: 10.1038/s41598-017-09666-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of photodestruction on 2^nd order SOFI calculations. (a) 2D plot of SNR and RMDS values of data with varying degrees of photodestruction. Photodestruction was simulated by assigning the emitters with a characteristic survival time (τ _bl). Datasets consisted of 500 frames (b) Effect of photodestruction on 2^nd order SOFI signal from different label densities. (c) Average 2^nd order SOFI images with varying degrees of photodestruction. Colour map: extended black body.