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. 2017 Sep 5;7:10470. doi: 10.1038/s41598-017-09666-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of different photodestruction-correcting methods on simulated data. (a) 2^nd order SOFI with fluorophores assigned a τ _bl value of 1.1 s. Batch sizes of 100, 50, 25, 10, and 5 frames were examined and are visualized by different marker sizes. (b) Close-up of the region in dotted box in (a). (c) Images of certain datasets to serve as a visual reference for RMSD values. (d–f) correspond to (a–c) respectively for 3^rd order simulations with a τ _bl value of 5.5 s. All images are averaged SOFI images of 100 repetitions. A batch size of 25 was used to generate the batching corrected image.