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. 2017 Sep 5;7:10522. doi: 10.1038/s41598-017-11274-1

Figure 4.

Figure 4

TRPV4-K535A channel loses activation by 5′,6′-EET not by heat or GSK1016790A agonist. (a) Changes in intracellular [Ca2+] (indicated by normalized fura-2 ratios) in HeLa cells transfected with GFP, TRPV4-WT or TRPV4-K535A cDNAs, after perfusion with 1 µM 5′,6′-EET or vehicle (as indicated). Traces are means ± SEM of 74–163 cells measured in 4–8 independent experiments. (b) Average [Ca2+]i increases (area under the curve) from traces shown in a. Numbers inside the bars indicate the number of cells analyzed. (c) Changes in intracellular [Ca2+] in response to warm solution (38 °C). (d) Average [Ca2+]i increases (area under the curve) from traces shown in c. (e) Changes in intracellular [Ca2+] in response to 10 nM GSK1016790A (GSK). Traces are means of 93–109 cells measured in 4–6 independent experiments. *P < 0.05 or not significant (n.s.) when compared with cells expressing TRPV4 WT channels (Kruskal-Wallis followed by Dunn post hoc test).