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. 2017 Sep 5;7:10522. doi: 10.1038/s41598-017-11274-1

Figure 5.

Figure 5

Effect of K535A substitution on the activation of TRPV4 whole-cell currents. Representative ramp current desnsity-voltage relations of whole-cell cationic currents recorded from HEK293 cells transfected with either TRPV4 WT or TRPV4 K535A cDNAs before (control) and after dialysis with 500 nM 5′,6′-EET (a), exposition to a 30% hypotonic shock (c) or to 10 nM GSK1016790A (e). (b,d,f) Average TRPV4 current density (at + 100 mV) increases in response to the above indicated stimuli. Data are expressed as the mean ± SEM, and the number of cells recorded is shown for each experimental condition. **P < 0.01, *P < 0.05 K535A versus WT. P values in panel b = 0.007, panel d = 0.002 and > 0.05 at al concentrations in panel f (One-tailed Student′s unpaired t test).