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. 2017 Sep 1;8:600. doi: 10.3389/fphar.2017.00600

Table 4.

Quantitative analysis for determination of phenolic components in the extracts (μg/g extract).

No. Phenolic compounds Extracts Analytical characteristics
Ethyl acetate Methanol Water Linear range (ppm) r2 LOD (ppm) LOQ (ppm)
1 Gallic acid 8 ± 0.4 100 ± 0.8 58 ± 0.8 0.20–25.0 0.9993 0.075 0.227
2 Protocatechuic acid 10 ± 0.4 80 ± 0.6 234 ± 8 0.20–25.0 0.9991 0.086 0.260
3 (+)-Catechin nd 152 ± 2 492 ± 20 0.90–113 0.9988 0.172 0.522
4 p-Hydroxybenzoic acid 44 ± 4 106 ± 4 200 ± 4 0.20–25.0 0.9994 0.007 0.020
5 Chlorogenic acid 210 ± 2 nd 166 ± 2 0.35–45.0 0.9988 0.080 0.241
6 Caffeic acid 42 ± 4 34 ± 4 90 ± 4 0.16–21.0 0.9993 0.054 0.162
7 (-)-Epicatechin 310 ± 16 950 ± 24 nd 0.50–66.0 0.9990 0.170 0.514
8 Syringic acid 22 ± 0.2 66 ± 4 86 ± 4 0.05–12.0 0.9995 0.030 0.090
9 Vanillin 2 ± 0.1 10 ± 0.2 nd 0.08–10.0 0.9995 0.020 0.060
10 p-coumaric acid 8 ± 0.6 34 ± 2 64 ± 2 0.04–6.0 0.9996 0.066 0.199
11 Ferulic acid 8 ± 0.4 90 ± 0.4 130 ± 6 0.12–17.0 0.9993 0.004 0.011
12 Sinapic acid nd nd 54 ± 2 0.12–17.0 0.9993 0.017 0.053
13 Benzoic acid 50 ± 0.6 50 ± 0.6 1386 ± 26 0.85–55.0 0.9998 0.111 0.335
14 o-Coumaric acid 82 ± 2 82 ± 2 nd 0.24–32.0 0.9988 0.023 0.069
15 Rutin nd nd nd 0.40–56.0 0.9989 1.113 3.373
16 Hesperidin nd nd nd 0.43–55.0 0.9992 1.080 3.280
17 Rosmarinic acid 76 ± 2 1960 ± 22 nd 0.02–7.0 0.9998 0.148 0.447
18 Eriodictyol nd nd 116 ± 4 0.33–21.0 0.9998 0.140 0.410
19 trans-Cinnamic acid 18 ± 1 66 ± 1.2 10 ± 1 0.02–7.0 0.9998 0.148 0.447
20 Quercetin nd nd 1356 ± 42 0.40–55.0 0.9999 0.013 0.040
21 Luteolin 756 ± 56 908 ± 56 1006 ± 56 0.13–17.0 0.9999 0.020 0.060
22 Kaempferol nd 288 ± 6 222 ± 4 0.05–15.0 0.9996 0.021 0.062
23 Apigenin 4136 ± 94 3314 ± 86 850 ± 24 0.17–11.0 0.9997 0.034 0.104

nd, not detected.