Table 7.
Primer pairs of the Cx43 for sequence, real-time PCR and plasmid construction.
| Primer Name | Sequence | Product Size (bp) |
|---|---|---|
| Cx43-P1-Fwd | 5′-ATCTCCACCATTCCCTTTGTTA-3′ | 150 |
| Cx43-P2-Rev | 5′-TGGGGAAGCATATAACCAGTTC-3′ | |
| hCx43-Fwd | 5′-CAGCTTGTACCCAGGAGGAG-3′ | 209 |
| hCx43-Rev | 5′TGTCCCTGGCCTTGAATATC-3′ | |
| hGAPDH-Fwd | 5′-CACCAGGTGGTCTCCTCTGAC-3′ | 150 |
| hGAPDH-Rev | 5′-GGTGGTCCAGGGGTCTTACTC-3′ | |
| hB2M-Fwd | 5′-CGCTACTCTCTCTTTCTGG-3′ | 165 |
| hB2M-Rev | 5′-TTCTCTCTCCATTCTTCAGTA-3′ | |
| hCx43-inject-Fwd | 5′- GATCCTCGAG TAGTTTTCTGGAGACATGGTAGAGG-3′ | 500 |
| hCx43-inject-Rev | 5′-GATCAGATCT TTACACTGCTTGCTTCACCAGA-3′ |