Table 2.
Thermal cycler conditions for 16 s rRNA gene amplification.
| Step | Temperature | Time | Repeats |
|---|---|---|---|
| 1 | 94 °C | 10 min | 1 |
| 2 | 94 °C | 30 sec | 30 |
| 3 | 55 °C | 30 sec | |
| 4 | 72 °C | 90 sec | |
| 5 | 72 °C | 7 min | 1 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.