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. 2017 Sep 5;7:10459. doi: 10.1038/s41598-017-11004-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cysteine and GSH levels were decreased in lysates of COS cells expressing 3MST in the presence of 3MP. (a) A representative HPLC chart of monobromobimane adducts of cell lysates. Cysteine and GSH were decreased in lysates of cells expressing 3MST compared with those transfected with an empty vector. Note that the reaction mixture of lysates contained approximately 1 μM cysteine and 10 μM GSH. (b,c) The levels of cysteine (b) and GSH (c) in cells expressing 3MST in the presence of 100 μM 3MP were significantly lower than those in control cells (n = 5). (d,e) Levels of cysteine (d) and GSH (e) in lysates of cells expressing various mutants of 3MST and rhodanese (Rhd) in the presence of 100 μM 3MP (n = 3). **p < 0.01, *p < 0.05 by ANOVA. All data expressed as mean ± s.e.m.