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. 2017 Sep 5;7:10578. doi: 10.1038/s41598-017-11030-5

Figure 3.

Figure 3

Formoterol (Form), but not clenbuterol (Clen), activates eNOS in a Gβγ- and Akt-dependent manner in RPTC. (A) p-eNOS was measured following 1 h of formoterol or clenbuterol. N = 5–6. (B) Phosphorylation of eNOS was measured following 30 min of treatment with formoterol in the presence or absence of the Gβγ inhibitor gallein (Gal) or the Akt inhibitor MK2206 (MK). N = 7–10. Images are cropped to show only relevant bands. Full sized images are available in Supplemental Figure 3. Mean + SEM. *p < 0.05 vs. DMSO, #p < 0.05 vs formoterol, one-way ANOVA with Sidak’s multiple comparison test.