Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 5;7:10577. doi: 10.1038/s41598-017-10918-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Orthogonally interacting SYNZIPs underlie the design of a protein nanotriangle. (A) Pairwise interaction profiles of SYNZIPs (1 to 6) determined by protein microarrays (in vitro, lower left)²⁰ and the yeast two-hybrid assays (in cells, upper right)¹⁹. Assay scores were normalized to a scale where 1.0 indicates tight binding. The corresponding K _D values are <10, <30, and <15 nM for 1:2, 3:4, and 5:6, respectively, and are approximated to be >400 nM for 3:5, and 4:6 ¹⁹. (B) Crystal structures of 1:2 (PDB ID: 3HE5) and 5:6 (PDB ID: 3HE4)²⁰, and a model of 3:4; melting temperatures are given in parentheses. (C) Topology of the designed heterotrimeric protein nanotriangle composed of the orthogonal SYNZIPs. (D) The composition of individual linked-SYNZIP fusion proteins that self-assemble into the nanotriangle shown in panel C. (E) A two-step thermal annealing process for assembly of the linked-SYNZIP fusion proteins, and illustration of desired assemblies at each step.