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. 2017 Sep 5;7:10442. doi: 10.1038/s41598-017-10905-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Uranyl acetate can be induced to fluoresce at cryogenic temperatures. (A) Emission spectra of uranyl acetate at temperatures between 21 °C and −195 °C. (B) Fluorescence microscopy images of Vero E6 cell sections prepared for TEM. The three sections were first imaged at 21 °C (i) before being cooled down to −195 °C and imaged again (ii), using identical illumination and acquisition parameters. The contrast of each panel is linearly stretched for comparison. (C) Comparing the uranyl acetate fluorescence signal from sections of Vero E6 cells imaged at 21 °C (i) and −195 °C (ii). (D and E) Line traces of the pixel intensities shown in (C i) (red) and (C ii) (blue). Absolute pixel values are shown in (D), and pixel values plotted on separate axes in (E). All scale bars represent 10 µm.