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. 2017 Sep 5;7:10428. doi: 10.1038/s41598-017-10699-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial spheroid invasion assays: (A) 3D cultured spheroid made of BxPC3, A375, M21 and BR-58 embedded in collagen type I after 3 and 12 hours (bar = 100 µm). (B) Illustration of transwell invasion assay. In this assay, spheroids are seeded onto collagen coated polycarbonate membrane. The upper chamber contains serum free media and the lower chamber is supplemented with 10% serum. After 48 hour invasive cells that have detached form the spheroids are stained and counted. (C) Quantification of cell invasion from spheroids of BxPC3, A375, M21 and BR-58 in transwell assay. n = 12, **p < 0.025, *p < 0.05.