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. 2017 Sep 5;8:427. doi: 10.1038/s41467-017-00573-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — STING activation induces apoptosis in T cells. a CD4⁺ T cells from wild-type (WT) or STING^gt/gt mice were treated with DMSO or 10-carboxymethyl-9-acridanone (CMA) for the indicated times and phosphorylation of TBK1 and NF-κB (p65) was assessed by immunoblot. b CD4⁺ T cells from WT or STING^gt/gt mice were treated with DMSO or CMA for 4 h and abundance of mRNAs of the indicated genes was measured by RT-qPCR. c Supernatants from CD4⁺ T cells or dendritic cells (DC) treated with DMSO or CMA were collected after 16 h. A bioassay was used to measure the production of type I IFNs (RLU relative light units). d, e CD4⁺ T cells from WT and STING^gt/gt mice were treated with DMSO or CMA. After 16 h cell viability d and percentage of apoptotic cells e, assessed by Annexin V staining, were determined. f WT CD4⁺ T cells were stimulated with CMA for the indicated times and caspases-3 cleavage was assessed by immunoblot. Quantification of cleaved Caspase-3 relative to β-Actin levels is shown on the right panel. Data are representative of n = 3 (a, f) independent experiments or mean and s.d. of technical replicates (n = 2) of one representative experiment out of n = 3 (b, d, e) or n = 2 (c) independent experiments are shown. Unprocessed original blots are shown in Supplementary Fig. 9.