Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 5;7:10504. doi: 10.1038/s41598-017-10692-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fold-change in mRNA expression of P0 vs E12.5 NDCs for selected developmental pathways and immunohistochemical analysis of component growth factors at E12.5 and P0. (A) Fold-change in mRNA expression of Shh signaling pathway elements showing significant downregulation at P0. (B) SHH protein staining showing intense positive staining of the notochord at E12.5 and diffuse staining of the NP at P0. (C) Fold-change in mRNA expression of TGF-β pathway elements showing significant upregulation of both Tgfb1 and Tgfbr2 at P0. (D) TGF-β1 protein staining showing few positively stained notochord cells at E12.5, and widespread positive staining of NP cells and diffuse NP ECM staining at P0. (E) Fold-change in mRNA expression of IGF pathway elements showing significant upregulation of Igf1 at P0. (F) IGF-1 protein staining showing positive staining of the notochord at E12.5, and widespread positive staining of NP cells and diffuse NP ECM staining at P0. N = 4; all fold changes p < 0.05, P0 compared to E12.5, analyzed by DESeq2 and adjusted for false discovery rate. Refer to Fig. 2 for annotation of structural regions.