Table 3.
Effects of individual and combined stresses of 2,4-D and salinity on glutathione reductase (GR), ascorbic acid (AsA), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), reduced glutathione (GSH), oxidized glutathione (GSSH) and ration of GSH/GSSH in the roots of two rice cultivars.
| Cultivar | Treatment | GR | ASA | DHAR | MDHAR | GSH | GSSG | GSH/ | Lignin (A280 mg −1 protein) |
|---|---|---|---|---|---|---|---|---|---|
| (U mg−1 protein) | (µg g−1 FW) | (U mg−1 protein) | (U mg−1 protein) | (nmol g−1 FW) | (nmol g−1 FW) | GSSG ratio | |||
| ZJ 88 | Control (CK) | 2.11 ± 0.16 f (100%) | 35.82 ± 2.36d (100%) | 11.21 ± 1.07bc (100%) | 3.80 ± 0.40c (100%) | 0.42 ± 0.02 cd (100%) | 0.117 ± 0.01bc (100%) | 3.68 ± 0.34ef (100%) | 7.43 ± 0.66 (100%) |
| T2 | 3.34 ± 0.39ef (58%) | 39.50 ± 1.49bcd (10%) | 12.60 ± 1.71abc (12%) | 6.77 ± 0.69ab (78%) | 0.49 ± 0.03abcd (17%) | 0.107 ± 0.01bc (−9%) | 4.61 ± 0.15bc (25%) | 8.05 ± 0.58 (8%) | |
| T3 | 3.04 ± 0.30ef (44%) | 38.12 ± 2.94 cd (6%) | 13.16 ± 1.13abc (17%) | 3.52 ± 0.55c (−7%) | 0.42 ± 0.01 cd (0%) | 0.137 ± 0.01ab (17%) | 3.15 ± 0.32fg (−14%) | 11.40 ± 0.87 (53%) | |
| T4 | 3.09 ± 0.42ef (46%) | 36.23 ± 3.88d (1%) | 15.82 ± 1.42a (41%) | 3.25 ± 0.58c (−14%) | 0.44 ± 0.02bcd (5%) | 0.137 ± 0.02ab (17%) | 3.24 ± 0.16fg (−12%) | 11.86 ± 0.96 (60%) | |
| T5 | 5.85 ± 0.52 cd (177%) | 42.86 ± 1.24ab (20%) | 12.97 ± 1.00abc (16%) | 5.22 ± 0.65bc (37%) | 0.50 ± 0.02abcd (19%) | 0.117 ± 0.01bc (0%) | 4.31 ± 0.27 cd (17%) | 14.20 ± 1.64 (91%) | |
| T6 | 4.22 ± 0.34de (100%) | 39.15 ± 1.60bcd (9%) | 12.78 ± 0.92abc (14%) | 8.19 ± 1.03a (116%) | 0.51 ± 0.04abc (21%) | 0.123 ± 0.01abc (5%) | 4.17 ± 0.41 cd (13%) | 14.64 ± 1.80 (97%) | |
| XS 134 | Control (CK) | 3.25 ± 0.20ef (100%) | 36.49 ± 2.14d (100%) | 10.66 ± 0.75c (100%) | 3.59 ± 0.26c (100%) | 0.40 ± 0.03d (100%) | 0.150 ± 0.02a (100%) | 2.68 ± 0.09 g (100%) | 8.10 ± 0.83 (100%) |
| T2 | 6.02 ± 0.77 cd (85%) | 39.82 ± 1.17bcd (9%) | 12.76 ± 0.75abc (20%) | 6.93 ± 0.31ab (93%) | 0.47 ± 0.02bcde (18%) | 0.113 ± 0.01bc (−24%) | 4.15 ± 0.23 cd (55%) | 10.23 ± 1.13 (26%) | |
| T3 | 6.56 ± 0.55bc (102%) | 40.71 ± 1.63abc (12%) | 13.79 ± 0.67abc (29%) | 4.09 ± 0.72c (14%) | 0.47 ± 0.02bcde (18%) | 0.120 ± 0.01abc (−20%) | 3.93 ± 0.16def (47%) | 10.63 ± 1.04 (31%) | |
| T4 | 8.15 ± 0.81ab (151%) | 43.72 ± 1.21ab (20%) | 14.38 ± 1.20ab (35%) | 4.40 ± 0.57c (23%) | 0.52 ± 0.03abc (30%) | 0.100 ± 0.01c (−33%) | 5.20 ± 0.42ab (94%) | 12.95 ± 0.89 (60%) | |
| T5 | 8.67 ± 0.33b (167%) | 45.49 ± 3.58a (25%) | 14.93 ± 1.31a (40%) | 6.53 ± 0.23ab (82%) | 0.55 ± 0.01a (38%) | 0.097 ± 0.001c (−35%) | 5.70 ± 0.11a (113%) | 17.67 ± 1.21 (118%) | |
| T6 | 10.48 ± 1.28a (223%) | 45.83 ± 2.64a (26%) | 15.47 ± 0.85a (45%) | 8.95 ± 0.87a (149%) | 0.54 ± 0.02ab (35%) | 0.093 ± 0.001c (−38%) | 5.80 ± 0.34a (116%) | 19.30 ± 1.04 (138%) |
Average ± standard error from four separate replicates. Values with different letters are significantly different at P ≤ 0.05 as determined by Duncan’s test. CK = control, T2 = Recommended dose of herbicide (2,4-D), T3 = 4 dS m−1, T4 = 8 dSm−1, T5 = 4 ds m−1 + Recommended dose of herbicide, and T6 = 8 dS m−1 + Recommended dose of herbicide. FW = Fresh weight, dS m−1 = deciSiemens per meter.