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. 2017 Sep 5;7:10431. doi: 10.1038/s41598-017-10651-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression levels of hepatic genes implicated in the regulation of the circulating Lyso-PL levels. (a) Lipases: Lipc, hepatic lipase; Lipg, endothelial lipase. (b) Phospholipases: Pla2g2a, phospholipase A₂ group IIA; Abhd3, abhydrolase domain-containing 3. (c) Acyltransferases: Lcat, lecithin-cholesterol acyltransferase; Lpcat3, lysophosphatidylcholine acyltransferase 3. (d) Pon1, Paraoxonase 1. The hamsters were assigned to six groups, depending on the pro-dyslipidemic treatment and duration of the experiment: control group-4 days (C-4d), high-fat diet-4 days (HFD-4d), pro-dyslipidemic agent-4 days (P407-4d), control group-30 days (C-30d), high-fat diet-30 days (HFD-30d), and pro-dyslipidemic agent-30 days (P407-30d). Total RNA was isolated from the liver and subjected to qRT-PCR analysis. The relative expression levels were determined using β-actin as the reference gene and were normalized to the C-4d group. The data are presented as means ± SEM (n = 9–10). The statistical comparisons among groups were conducted using two- and one-way ANOVAs. I: the effect of the intervention (HFD and P407); t: the effect of time; Ixt: the interaction between the two factors (two-way ANOVA, p < 0.05). For each gene, different superscript lowercase letters (^a,b,c) indicate significant different mean values (one-way ANOVA and Games-Howell post hoc test, p < 0.05).