Table 1.
Ionization mode | Ion m/z (MS) | Distinctive fragments (MS/MS) | Tentative identificationϮ | Lipid subclass | Increased with HFD feeding |
---|---|---|---|---|---|
+ESI | [M + H]+: 524.3743 | 184.1, 104.1, 60.1 | Lyso-PC (18:0) | Monoacylglycerophospho cholines | 15d |
[M + H]+: 520.3440 | 184.1, 104.1, 60.1 | Lyso-PC (18:2) | |||
[M + H]+: 518.3251 | 184.1, 104.1, 60.1 | Lyso-PC (18:3) | |||
[M + H]+: 550.3871 | 184.1, 104.1, 60.1 | Lyso-PC (20:1) | |||
[M + H]+: 548.3719 | 184.1, 104.1, 60.1 | Lyso-PC (20:2) | |||
−ESI | [M − H]−: 599.3218 | 419.2, 283.3, 79.0 | Lyso-PI (18:0) | Monoacylglycerophospho inositols | 30d |
[M − H]−: 597.3060 | 417.2, 281.8, 79.0 | Lyso-PI (18:1) | |||
[M − H]−: 621.3060 | 441.0, 305.1, 79.0 | Lyso-PI (20:3) | |||
[M − H]−: 619.2906 | 439.0, 303.2, 79.0 | Lyso-PI (20:4) |
ϮAccording to the LIPID MAPS32 glycerophospholipid nomenclature, the structure of the acyl chain is indicated within parentheses in the ‘Headgroup (sn1 or sn2)’ format. The headgroup is assumed to be attached to the sn3 position of glycerol.
Abbreviations: Lyso-PC, lysophosphatidylcholine; Lyso-PI, lysophosphatidylinositol.