Figure 4.

Whole mount in situ hybdridisation showing Fgf-3 and Sox6 expression in mouse embryos. Mouse embryos (E9.5) were hybridised with either antisense Fgf-3 (A and B) or antisense Sox6 (C and D) probes labelled with digoxigenin and detected with anti-digoxigenin alkaline phosphatase conjugated antiserum as described in Materials and Methods. The major common expression site is in the otic vesicle (OV). Sections through the otic vesticles seen in (A) and (D) are shown in (B) and (C), respectively, and demonstrate the cellular location of the labelled cells.