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Interaction of SOX6 with CtBP2 in yeast depends on the PLNLSS sequence motif in SOX6. (A) Amino acid sequences of the putative recognition site for CtBP2 in wild-type SOX6 and mutated SOX6. (B) HF7c yeast cells were co-transformed with plasmids expressing wild-type or a mutant GAL4DBD-Sox6 and GAL4AD-CtBP2. For each transformation single colonies were tested for growth on His– plates (left) and for β-galactosidase activity (right), respectively.
