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. 2001 Aug 15;29(16):3347–3355. doi: 10.1093/nar/29.16.3347

Figure 6.

Figure 6

Interaction of SOX6 with CtBP2 in Cos7 cells depends on the PLNLSS sequence in SOX6. Histidine-tagged SOX6 and FLAG-tagged CtBP2 were co-expressed in Cos7 cells. Lanes 1 and 5, SOX6 wild-type; lanes 2 and 6, SOX6del; lanes 3 and 7, SOX6P383A; lanes 4 and 8, SOX6L386H (see Fig. 5 for mutated amino acid sequences). (A) Total cell lysates (lanes 5–8) or cell lysates immunoprecipitated with anti-FLAG serum (lanes 1–4) were analysed by immunoblotting with ant-His serum. (B) As described in (A) except cell lysates were immunoprecipitated with anti-His serum and immunoblotted with anti-FLAG serum.