Fig. 1.

PTEN-loss carcinogenesis is associated with transcriptional upregulation of LXR target genes. a ONCOMINE boxed plot of PTEN, FASN and APOE expression levels between human prostate gland, prostate carcinoma and metastasis in various human data sets^{2, 57, 58}. b Heatmap of LXR target gene signature and PTEN expression in Grasso et al.⁵⁷ data set. c Gene Set Enrichment Analysis of LXR target gene signature based on PTEN status using Grasso et al.⁵⁷ data set. d HE staining and immunohistofluorescence against PTEN, AKTS473 and ABCA1 of wild type (WT) and Pten ^{pc −/−} mouse prostate tissues from 6-month-old animals. Scale bar, 100 µm. Nuclei are stained using Hoescht (blue). e Western blot analysis of PTEN, AKTS473, AKT, FASN, APOE and GAPDH of wild type (WT), Pten ^{pc −/−} and Pten ^{pc −/−} lxrαβ ^−/− mouse prostatic tissues from 6-month-old animals. f–h Relative expression of LXR targets genes, non-LXR target gene panel, Lxrα (Nr1h3) and Lxrβ (Nr1h2). All data are represented as mean ± SEM and statistical analyses were performed with the Student’s t-test; *p < 0.05, **p < 0.01 and ***p < 0.001. See also Supplementary Figs. 1 and 2