Fig. 6.

LXRs control invasiveness of tumor cells through upregulation of EMT and metalloproteases. a, b Relative expression and protein accumulation of EMT markers in prostate from wild type (WT), Pten ^{pc −/−} and Pten ^{pc −/−} lxrαβ ^−/− mice (N = 10/7/8). c Immunofluorescence staining against EMT markers VIMENTIN and SNAI, together with Ki67. Nuclei are stained using Hoescht (blue). d Relative expression analysis of metalloproteinase genes Mmp1, 2, 7, 9 and negative regulator Timp2 in prostate from wild type (WT), Pten ^{pc −/−} and Pten ^{pc −/−} lxrαβ ^−/− mice (N = 10 per group). e MMP9 protein accumulation in prostate from wild type (WT), Pten ^{pc −/−} and Pten ^{pc −/−} lxrαβ ^−/− mice. f Histological and immunofluorescence staining for MMP9 in lung from Pten ^{pc −/−} and Pten ^{pc −/−} lxrαβ ^−/− mice. White arrows indicate the positive MMP9 staining surrounded metastatic cells in contact with matrix. Asterisks identify metastatic cells and black arrows the surrounded pneumocytes. Nuclei are stained using Hoescht (blue). All data are represented as mean ± SEM and statistical analyses were performed with the Student’s t-test; **p < 0.01; ***p < 0.001. See also Supplementary Fig. 13